HPRT
(Plasmid #22884)

• Depositing Lab: James Thomson
• Publication: Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Backbone

• Vector backbone: pBluescript SK (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 9000
• Vector type: Cre/Lox ; homologous recombination

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hypoxanthine phosphoribosyltransferase-1
• Alt name: HPRT; HGPRT; HPRT1
• Species: H. sapiens (human)
• Insert Size (bp): 11900
• Mutation: PGK, NeoR, and BGH polyA are between the two genomic sequences (one section is 9.1kb and one is 2.5kb).
• GenBank ID: NC_000023
• Entrez Gene: HPRT1 (a.k.a. HGPRT, HPRT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI and XmaI (not destroyed)
• 3′ cloning site: EcoRI and NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pLac

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that very little of the insert has been sequenced by Addgene. Please be sure to do your own sequencing of HPRT before using this plasmid.