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Addgene

pCIG-VP16 RARa [TJ#389]
(Plasmid #16287)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16287 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCIG
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAR alpha
  • Alt name
    RARa
  • Alt name
    retinoic acid receptor, alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Entrez Gene
    RARA (a.k.a. NR1B1, RAR, RARalpha)
  • Tag / Fusion Protein
    • VP16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VP16-RARalpha. Note that this contains the 3'UTR of RARalpha, though no polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG-VP16 RARa [TJ#389] was a gift from Thomas Jessell (Addgene plasmid # 16287 ; http://n2t.net/addgene:16287 ; RRID:Addgene_16287)
  • For your References section:

    A requirement for retinoic acid-mediated transcriptional activation in ventral neural patterning and motor neuron specification. Novitch BG, Wichterle H, Jessell TM, Sockanathan S. Neuron. 2003 Sep 25. 40(1):81-95. 10.1016/j.neuron.2003.08.006 PubMed 14527435