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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIG
- Backbone size w/o insert (bp) 6200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAR alpha
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Alt nameRARa
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Alt nameretinoic acid receptor, alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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Entrez GeneRARA (a.k.a. NR1B1, RAR, RARalpha)
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Tag
/ Fusion Protein
- VP16 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
VP16-RARalpha. Note that this contains the 3'UTR of RARalpha, though no polyA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG-VP16 RARa [TJ#389] was a gift from Thomas Jessell (Addgene plasmid # 16287 ; http://n2t.net/addgene:16287 ; RRID:Addgene_16287) -
For your References section:
A requirement for retinoic acid-mediated transcriptional activation in ventral neural patterning and motor neuron specification. Novitch BG, Wichterle H, Jessell TM, Sockanathan S. Neuron. 2003 Sep 25. 40(1):81-95. 10.1016/j.neuron.2003.08.006 PubMed 14527435
Map uploaded by the depositor.