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Addgene

FIRES-PBCS-CLV3
(Plasmid #228697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    nonspecific
  • Vector type
    Mammalian Expression, Synthetic Biology, Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FIRE1 SENSOR
  • Species
    Synthetic
  • Promoter hpgk
  • Tag / Fusion Protein
    • mClover3

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FIRES-PBCS-CLV3 was a gift from Carolyn Sangokoya (Addgene plasmid # 228697 ; http://n2t.net/addgene:228697 ; RRID:Addgene_228697)

  • For your References section:

    The FIRE biosensor illuminates iron regulatory protein activity and cellular iron homeostasis. Sangokoya C. Cell Rep Methods. 2025 Jan 11:100960. doi: 10.1016/j.crmeth.2024.100960. 10.1016/j.crmeth.2024.100960 PubMed 39824193
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