pCph8
(Plasmid
#22869)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPROTet
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 2021
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsEnvZ knockout strain
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCph8
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SpeciesSynechocystis/E.coli fusion gene
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Insert Size (bp)2236
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MutationFirst 517 amino acids of Cph1 and the last 229 amino acids of EnvZ. ILE TO VAL mutation at position 136 of the HK EnvZ domain.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCph8 was a gift from Christopher Voigt (Addgene plasmid # 22869 ; http://n2t.net/addgene:22869 ; RRID:Addgene_22869) -
For your References section:
Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24. 438(7067):441-2. 10.1038/nature04405 PubMed 16306980