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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6-natMX6
- Backbone size w/o insert (bp) 3697
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Vector typeYeast Expression
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Selectable markersNatamycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeroGFP & UPR-RFP
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Insert Size (bp)3253
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (?) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byroGFP is from SJ Remington at U. Oregon
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPM48 was a gift from Feroz Papa (Addgene plasmid # 22854 ; http://n2t.net/addgene:22854 ; RRID:Addgene_22854) -
For your References section:
Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions. Merksamer PI, Trusina A, Papa FR. Cell. 2008 Nov 28. 135(5):933-47. 10.1016/j.cell.2008.10.011 PubMed 19026441