pChillyBoys_gen1
(Plasmid #228495)

• Purpose: pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
• Depositing Lab: Richard Baker
• Publication: Molecular Toolbox (unpublished)

Backbone

• Vector backbone: pACYCDuet-1
• Backbone size w/o insert (bp): 3700
• Total vector size (bp): 5810
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: groEL
• Alt name: Chaperonin GroEL
• Alt name: CH60_OLEAN
• Species: Oleispira antarctica
• Insert Size (bp): 1611
• Promoter: T7 Promoter

Cloning Information for Gene/Insert 1

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Gene/Insert 2

• Gene/Insert name: groES
• Alt name: Co-chaperonin GroES
• Alt name: CH10_OLEAN
• Species: Oleispira antarctica
• Insert Size (bp): 291
• Promoter: T7 Promoter

Cloning Information for Gene/Insert 2

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • 4XJNZK_2_MZB214_2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IPTG inducible expression of Oleispira antarctica GroEL and GorES, cold adapted chaperonins to assist in protein folding at lower expression temperatures