pRS300
(Plasmid #22846)

• Purpose: Backbone for expressing plant artificial miRNAs
• Depositing Lab: Detlef Weigel
• Publication: Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

Backbone

• Vector backbone: pBluescriptSK+
• Backbone size w/o insert (bp): 2964
• Vector type: Plant Expression, RNAi ; Plant gene inactivation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MIR319a
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 404
• Entrez Gene: MIR319a (a.k.a. AT4G23713, JAGGED AND WAVY, JAW, MICRORNA 319, MIR319, MIR319A, microRNA319A, p_MI0000544)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sma1 (destroyed during cloning)
• 3′ cloning site: Sma1 (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See http://wmd3.weigelworld.org/

for more information