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Addgene

AP1muA-TurboID-polyA-G418 HR
(Plasmid #227734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIST Amp high copy
  • Backbone manufacturer
    TWIST
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    genomic sequence AP1M1 locus
  • Species
    H. sapiens (human)
  • Entrez Gene
    AP1M1 (a.k.a. AP47, CLAPM2, CLTNM, MU-1A, mu1A)
  • Tag / Fusion Protein
    • Turbo-ID, V5

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AP1muA-TurboID-polyA-G418 HR was a gift from Francesca Bottanelli (Addgene plasmid # 227734 ; http://n2t.net/addgene:227734 ; RRID:Addgene_227734)

  • For your References section:

    When less is more - a fast TurboID knock-in approach for high-sensitivity endogenous interactome mapping. Stockhammer A, Spalt C, Klemt A, Benz LS, Harel S, Natalia V, Wiench L, Freund C, Kuropka B, Bottanelli F. J Cell Sci. 2024 Aug 15;137(16):jcs261952. doi: 10.1242/jcs.261952. Epub 2024 Aug 28. 10.1242/jcs.261952 PubMed 39056144
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