Skip to main content
Addgene

pMyt1a
(Plasmid #22695)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT7T3
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 2883
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human Myelin Transcription Factor 1
  • Alt name
    Myt1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    MYT1 (a.k.a. MTF1, MYTI, PLPB1, C20orf36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3 Promoter
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Human Myt1 cDNA was cloned in Hudson lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Myt1 DNA was screened from a fetal brain expression library. It contains the carboxy but not the amino terminus of the protein. It is a partial cDNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyt1a was a gift from Lynn Hudson (Addgene plasmid # 22695 ; http://n2t.net/addgene:22695 ; RRID:Addgene_22695)

  • For your References section:

    Novel member of the zinc finger superfamily: A C2-HC finger that recognizes a glia-specific gene. Kim JG, Hudson LD. Mol Cell Biol. 1992 Dec . 12(12):5632-9. PubMed 1280325
Related items:
Commonly requested with: