SigmaR1-mNeonGreen
(Plasmid
#226566)
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PurposeEncodes SigmaR1 protein labeled with mNeonGreen
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemNeonGreen-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSigmaR1 (Sigma-1 Receptor)
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SpeciesH. sapiens (human)
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Entrez GeneSIGMAR1 (a.k.a. ALS16, DSMA2, OPRS1, SIG-1R, SR-BP, SR-BP1, SRBP, hSigmaR1, sigma1R)
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Tag
/ Fusion Protein
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SigmaR1-mNeonGreen was a gift from Gia Voeltz (Addgene plasmid # 226566 ; http://n2t.net/addgene:226566 ; RRID:Addgene_226566) -
For your References section:
SigmaR1 shapes rough endoplasmic reticulum membrane sheets. Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, Voeltz GK. Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 10.1016/j.devcel.2024.06.005. 10.1016/j.devcel.2024.06.005 PubMed 38971154