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Addgene

pCyCBE
(Plasmid #226505)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226505 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    RSF1010
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    APOBEC1-Cas9D10A
  • Insert Size (bp)
    4839

Gene/Insert 2

  • Gene/Insert name
    sgRNA

Gene/Insert 3

  • Gene/Insert name
    sacB gene

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCyCBE was a gift from Weizhong Chen (Addgene plasmid # 226505 ; http://n2t.net/addgene:226505 ; RRID:Addgene_226505)
  • For your References section:

    Development of a base editor for convenient and multiplex genome editing in cyanobacteria. Li XD, Liu LM, Xi YC, Sun QW, Luo Z, Huang HL, Wang XW, Jiang HB, Chen W. Commun Biol. 2024 Aug 14;7(1):994. doi: 10.1038/s42003-024-06696-3. 10.1038/s42003-024-06696-3 PubMed 39143188