pCyCBE
(Plasmid
#226505)
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PurposeA cytidine deaminase-mediated base-editing plasmid in cyanobacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRSF1010
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Vector typeCRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameAPOBEC1-Cas9D10A
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Insert Size (bp)4839
Gene/Insert 2
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Gene/Insert namesgRNA
Gene/Insert 3
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Gene/Insert namesacB gene
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCyCBE was a gift from Weizhong Chen (Addgene plasmid # 226505 ; http://n2t.net/addgene:226505 ; RRID:Addgene_226505) -
For your References section:
Development of a base editor for convenient and multiplex genome editing in cyanobacteria. Li XD, Liu LM, Xi YC, Sun QW, Luo Z, Huang HL, Wang XW, Jiang HB, Chen W. Commun Biol. 2024 Aug 14;7(1):994. doi: 10.1038/s42003-024-06696-3. 10.1038/s42003-024-06696-3 PubMed 39143188