phGf
(Plasmid #22516)

• Purpose: (Empty Backbone)
• Depositing Lab: Christopher Buck
• Publication: Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Backbone

• Vector backbone: Manufactured in our lab
• Backbone size (bp): 4508
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdb
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: chloramphenicol resistance and CcdB gene
• Alt name: CAT
• Alt name: control of cell division or death
• Insert Size (bp): 1100

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (destroyed during cloning)
• 3′ cloning site: attR2 (destroyed during cloning)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

First reference to this plasmid was in:

Buck, C.B., et al., Human alpha-defensins block papillomavirus infection. Proc Natl Acad Sci U S A, 2006. 103(5): p. 1516-21.

Genbank style annotated sequence can be found at:
http://home.ccr.cancer.gov/Lco/support.htm