GST-Hsp90 M(272-617)
(Plasmid #22482)

• Purpose: Please note this plasmid contains GST-Hsp90 residues 273-617.
• Depositing Lab: William Sessa
• Publication: Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Backbone

• Vector backbone: pGEX-4T3
• Backbone size w/o insert (bp): 4968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hsp90alpha M domain
• Alt name: Hsp90 M(273-617)
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Entrez Gene: HSP90AA2P (a.k.a. HSP90AA2, HSP90ALPHA, HSPCA, HSPCAL3)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pGEX5'

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry