FUtdTW
(Plasmid #22478)

• Depositing Lab: Connie Cepko
• Publication: Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: I. Verma, Salk
• Backbone size w/o insert (bp): 9165
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tandem dimer tomato
• Alt name: tdtomato
• Alt name: tomato
• Species: Discosoma sp
• Insert Size (bp): 1400
• Promoter: UbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI, BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: hUBCpro-F (5'-TGAAGCTCCGGTTTTGAACT-3')
• 3′ sequencing primer: WPRE-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pFUGW-tdtomato