Skip to main content
Addgene

EGFP-INPP5E
(Plasmid #223564)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INPP5E
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Inpp5e (a.k.a. 1200002L24Rik, 72kDa, mKIAA0123)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspE1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer not sure
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-INPP5E was a gift from Pietro De Camilli (Addgene plasmid # 223564 ; http://n2t.net/addgene:223564 ; RRID:Addgene_223564)

  • For your References section:

    Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary properties in iPSC-derived dopaminergic neurons. Mohd Rafiq N, Fujise K, Rosenfeld MS, Xu P, De Camilli P. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2318943121. doi: 10.1073/pnas.2318943121. Epub 2024 Apr 18. 10.1073/pnas.2318943121 PubMed 38635628
Related items: