CMV-SNAP-hCB1_IRES_EGFP
(Plasmid #223505)

• Purpose: Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
• Depositing Lab: James Frank
• Publication: Frank Lab Plasmids 2024 (unpublished)

Backbone

• Vector backbone: pIRES2
• Backbone manufacturer: Clontech (TaKaRa)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNAP-tag-CNR1 and EGFP
• Species: H. sapiens (human), Synthetic
• Entrez Gene: CNR1 (a.k.a. CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR)
• Tag / Fusion Protein:
  • SNAP-tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • CMV-SNAP-hCB1_IRES_EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry