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pFastbac-Flag-BRCA1
(Plasmid #223228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastbac
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 10389
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Breast Cancer 1
  • Alt name
    BRCA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5589
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastbac-Flag-BRCA1 was a gift from Weixing Zhao (Addgene plasmid # 223228 ; http://n2t.net/addgene:223228 ; RRID:Addgene_223228)

  • For your References section:

    Crucial roles of the BRCA1-BARD1 E3 ubiquitin ligase activity in homology-directed DNA repair. Wang M, Li W, Tomimatsu N, Yu CH, Ji JH, Alejo S, Witus SR, Alimbetov D, Fitzgerald O, Wu B, Wang Q, Huang Y, Gan Y, Dong F, Kwon Y, Sareddy GR, Curiel TJ, Habib AA, Hromas R, Dos Santos Passos C, Yao T, Ivanov DN, Brzovic PS, Burma S, Klevit RE, Zhao W. Mol Cell. 2023 Oct 19;83(20):3679-3691.e8. doi: 10.1016/j.molcel.2023.09.015. Epub 2023 Oct 4. 10.1016/j.molcel.2023.09.015 PubMed 37797621
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