pTT3-ecdCD19-ST3-His_withSUMO
(Plasmid #223219)

• Purpose: Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression
• Depositing Lab: Omer Dushek
• Publication: Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.1038/s44318-023-00012-1. Epub 2023 Dec 18.

Backbone

• Vector backbone: pTT3
• Backbone size w/o insert (bp): 6107
• Total vector size (bp): 7202
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: extracellular domain of B-lymphocyte antigen CD19
• Alt name: B-lymphocyte surface antigen B4
• Alt name: Differentiation antigen CD19
• Alt name: T-cell surface antigen Leu-12
• Species: H. sapiens (human)
• Insert Size (bp): 777
• GenBank ID: NP_001171569.1 NM_001178098.1
• Entrez Gene: CD19 (a.k.a. B4, CVID3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Histag (C terminal on insert)
  • Spytag (C terminal on insert)
  • SUMO (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gttggggtgagtactccc

Resource Information

• Supplemental Documents:
  • pTT3-ecdCD19-ST3-His_withSUMO.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry