pMZ7
(Plasmid
#223180)
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Purpose(Empty Backbone) pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223180 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMZ7
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Vector typeChromosomal integration
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin (200 μg/mL), Kanamycin (50 μg/mL)
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Growth Temperature37°C
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Growth Strain(s)EC1000, Bacterial strain #71852
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Growth instructions40 μg/mL Kanamycin required for EC1000
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Please visit https://doi.org/10.1101/2023.12.30.573720 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMZ7 was a gift from Paul Blainey (Addgene plasmid # 223180 ; http://n2t.net/addgene:223180 ; RRID:Addgene_223180) -
For your References section:
Vaginal Lactobacillus fatty acid response mechanisms reveal a novel strategy for bacterial vaginosis treatment. Zhu M, Frank MW, Radka CD, Jeanfavre S, Tse MW, Pacheco JA, Pierce K, Deik A, Xu J, Hussain S, Hussain FA, Xulu N, Khan N, Pillay V, Dong KL, Ndung'u T, Clish CB, Rock CO, Blainey PC, Bloom SM, Kwon DS. bioRxiv [Preprint]. 2023 Dec 30:2023.12.30.573720. doi: 10.1101/2023.12.30.573720. 10.1101/2023.12.30.573720 PubMed 38234804
