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pMAL-7xHis-TEV-hexa
(Plasmid #222868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c2
  • Backbone manufacturer
    New England Biolabs
  • Total vector size (bp) 7390
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BL21-RIL
  • Growth instructions
    Recommended growth temperature of 25ºC for protein production. Chloramphenicol at 30 μg/mL should be included in media when using BL21-RIL cells to maintain the additional plasmid (pRIL) that provides rare tRNAs.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    tobacco etch virus
  • Mutation
    C19V, L56V, C110V, C130S, S135G, S219V. Insert includes a TEV cleavage site between the MBP tag and the 7xHis tag.
  • GenBank ID
    M11458.1
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • 7xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13-48REV
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Waugh. The plasmid with the gene for the TEV-Triple mutant (L56V, S135G, S219V) was received from Addgene (pDZ2087, Plasmid #92414) and was originally deposited by the David Waugh Lab. The three additional mutations in this version of TEV (C19V, C110V, C130S) were added via site-directed mutagenesis.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations based on:
Correnti et al., 2018. Nature Structure and Molecular Biology. https://doi.org/10.1038/s41594-018-0033-9
Cabrita et al., 2007. Protein Science. https://doi.org/10.1110/ps.072822507
Kapust et al., 2001. Protein Engineering. https://doi.org/10.1093/protein/14.12.993

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-7xHis-TEV-hexa was a gift from Nikolaus Loening (Addgene plasmid # 222868 ; http://n2t.net/addgene:222868 ; RRID:Addgene_222868)

  • For your References section:

    An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents. Davis SM, Romig BL, Abe AA, Loening NM. Protein Sci. 2025 Mar;34(3):e70049. doi: 10.1002/pro.70049. 10.1002/pro.70049 PubMed 39969093
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