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Addgene

pFast-Flag-hRad9
(Plasmid #222468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 6861
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hRad9
  • Alt name
    Human cell cycle checkpoint protein Rad9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2086
  • Entrez Gene
    RAD9A (a.k.a. RAD9)
  • Tag / Fusion Protein
    • Flag epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hRad9 was amplified by PCR with pcDNA3-AU1-hRAD9, a kind gift of Larry M. Karnitz (Mayo Foundation, Rochester, MN), as the template

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFast-Flag-hRad9 was a gift from Aziz Sancar (Addgene plasmid # 222468 ; http://n2t.net/addgene:222468 ; RRID:Addgene_222468)

  • For your References section:

    Purification and characterization of human DNA damage checkpoint Rad complexes. Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11236-41. doi: 10.1073/pnas.201373498. 10.1073/pnas.201373498 PubMed 11572977
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