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Addgene

pcDNA3-HA-Epsin 2
(Plasmid #22241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epsin 2
  • Alt name
    EH domain-binding protein
  • Alt name
    EHB21
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • GenBank ID
    AF062085
  • Entrez Gene
    EPN2 (a.k.a. EHB21)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-Epsin 2 was a gift from Pietro De Camilli (Addgene plasmid # 22241 ; http://n2t.net/addgene:22241 ; RRID:Addgene_22241)

  • For your References section:

    The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module. Rosenthal JA, Chen H, Slepnev VI, Pellegrini L, Salcini AE, Di Fiore PP, De Camilli P. J Biol Chem. 1999 Nov 26. 274(48):33959-65. 10.1074/jbc.274.48.33959 PubMed 10567358
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