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pCF1148
(Plasmid #222330)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222330 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBSJL2
  • Backbone size w/o insert (bp) 7769
  • Modifications to backbone
    The tetM and bla antibiotic resistance cassettes and f1 phage origin were replaced with the cat gene and promoter from pRPF185 (Addgene #106367).
  • Vector type
    Bacterial Expression ; E. coli-Veillonella shuttle plasmid
  • Selectable markers
    select with chloramphenicol analog thiamphenicol (15 µg/ml) in Veillonella

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    bs2 gene with Veillonella parvula SKV38 mdh promoter
  • Alt name
    FbFP, flavin mononucleotide-binding fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter Veillonella parvula SKV38 mdh promoter

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer CTCATACTAGTGGATCCAAAAATACCAAAATTCTTCAAAAAAATC
  • 3′ sequencing primer GATATTGTGTCCTGGGATCCCTGCAGTTATTCTAATAATTTTTC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Bs2 gene was originally described in Drepper, T., et al. (2007) Reporter proteins for in vivo fluorescence without oxygen. Nature biotechnology 25, 443-445.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF1148 was a gift from J Christopher Fenno (Addgene plasmid # 222330 ; http://n2t.net/addgene:222330 ; RRID:Addgene_222330)

  • For your References section:

    Development of a small shuttle plasmid for use in oral Veillonella and initial appraisal of potential for fluorescence-based applications. Goetting-Minesky MP, Kim J, White DT, Hayashi M, Rickard AH, Fenno JC. Lett Appl Microbiol. 2024 Aug 5;77(8):ovae069. doi: 10.1093/lambio/ovae069. 10.1093/lambio/ovae069 PubMed 39020263
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