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FusX alphaDdCBE DddA6 1397N
(Plasmid #221939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size (bp) 4717
  • Vector type
    Synthetic Biology
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I, T1380I
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Part of the FusX TALE Base Editor (FusXTBE) platform. Includes synthetic COX8A MTS-3xFLAG-TALE NT-alphaN*-lacZalpha-TALE CTD*-DddA6 1397N-UGI-ATP5B 3'UTR (1844 bp). 5' Cloning Site: SacI (Not Destroyed), 3' Cloning Site: PstI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FusX alphaDdCBE DddA6 1397N was a gift from Stephen Ekker (Addgene plasmid # 221939 ; http://n2t.net/addgene:221939 ; RRID:Addgene_221939)

  • For your References section:

    Unconstrained Precision Mitochondrial Genome Editing with alphaDdCBEs. Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC. Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073. 10.1089/hum.2024.073 PubMed 39212664
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