pEBB-GFP-hBEN
(Plasmid #22157)

• Depositing Lab: Ananda Roy
• Publication: Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98(14):7789-94.

Backbone

• Vector backbone: pEBB
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hBEN
• Alt name: MusTRD1
• Species: H. sapiens (human)
• Insert Size (bp): 2900
• Entrez Gene: GTF2IRD1 (a.k.a. BEN, CREAM1, GTF3, MUSTRD1, RBAP2, WBS, WBSCR11, WBSCR12, hMusTRD1alpha1)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homologous to mouse 1alpha1 isoform
GFP cloned in SpeI/ClaI