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Addgene

HyperNova/pRSETB
(Plasmid #221456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRESTB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HyperNova
  • Alt name
    HN
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • GenBank ID
    LC771173

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that older versions of Snapgene's software may annotate "HyperNova" incorrectly as "SuperNova". We have verified that this plasmid encodes HyperNova. Please see the depositor's annotated file under "Supplemental Information" for additional annotations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HyperNova/pRSETB was a gift from Kiwamu Takemoto (Addgene plasmid # 221456 ; http://n2t.net/addgene:221456 ; RRID:Addgene_221456)

  • For your References section:

    Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova. Shidara H, Shirai T, Ozaki-Noma R, Jitsuki S, Nagai T, Takemoto K. Commun Biol. 2024 Aug 6;7(1):945. doi: 10.1038/s42003-024-06583-x. 10.1038/s42003-024-06583-x PubMed 39107369
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