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Addgene

pCRISPaint-APEX2-GFP-3xFLAG-PuroR
(Plasmid #221238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPaint
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APEX2-GFP-3xFLAG
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPaint-APEX2-GFP-3xFLAG-PuroR was a gift from Jonathan Kagan (Addgene plasmid # 221238 ; http://n2t.net/addgene:221238 ; RRID:Addgene_221238)

  • For your References section:

    Molecular definition of the endogenous Toll-like receptor signalling pathways. Fisch D, Zhang T, Sun H, Ma W, Tan Y, Gygi SP, Higgins DE, Kagan JC. Nature. 2024 Jul;631(8021):635-644. doi: 10.1038/s41586-024-07614-7. Epub 2024 Jul 3. 10.1038/s41586-024-07614-7 PubMed 38961291
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