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Addgene

pWJPrP5
(Plasmid #22106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBTIIKS(+)
  • Backbone size w/o insert (bp) 2900
  • Vector type
    construction of targeting vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse PrP ORF insert
  • Alt name
    PrP-D177N
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    800
  • Mutation
    mutated codon 177 (D to N)
  • Entrez Gene
    Prnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eag1 (not destroyed)
  • 3′ cloning site Cla1 (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWJPrP5 was a gift from Susan Lindquist (Addgene plasmid # 22106 ; http://n2t.net/addgene:22106 ; RRID:Addgene_22106)

  • For your References section:

    Spontaneous generation of prion infectivity in fatal familial insomnia knockin mice. Jackson WS, Borkowski AW, Faas H, Steele AD, King OD, Watson N, Jasanoff A, Lindquist S. Neuron. 2009 Aug 27. 63(4):438-50. 10.1016/j.neuron.2009.07.026 PubMed 19709627
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