pSLIK 3xFLAG-NUP37 neo
(Plasmid
#220361)
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PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSLIK neo
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Backbone manufacturerAddgene #25735
- Backbone size w/o insert (bp) 13286
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUP37
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Alt nameMCPH24
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SpeciesH. sapiens (human)
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Entrez GeneNUP37 (a.k.a. MCPH24, p37)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK 3xFLAG-NUP37 neo was a gift from Kevin Janes (Addgene plasmid # 220361 ; http://n2t.net/addgene:220361 ; RRID:Addgene_220361)