pKI_ΩBBMVP16&SRDXWUSm1
(Plasmid #220349)

• Purpose: Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
• Depositing Lab: Tomoko Igawa
• Publication: Sato et al Front Plant Sci. 2024 Apr 3;15:1308417. doi: 10.3389/fpls.2024.1308417. eCollection 2024.

Backbone

• Vector backbone: pKI1.1R
• Total vector size (bp): 14773
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BABY BOOM
• Species: A. thaliana (mustard weed)
• Entrez Gene: BBM (a.k.a. AT5G17430, BABY BOOM, T10B6.90, T10B6_90)
• Promoter: CaMV 35S
• Tag / Fusion Protein:
  • VP16 transcriptional activation domain (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aacagtaccctccggcaacatc

Gene/Insert 2

• Gene/Insert name: WUSCHEL
• Species: A. thaliana (mustard weed)
• Mutation: two amino acid substitution in WUS-box: L255A L257A
• Entrez Gene: WUS (a.k.a. AT2G17950, PGA6, T27K22.18, T27K22_18, WUS1, WUSCHEL, WUSCHEL 1)
• Promoter: RPS5A
• Tag / Fusion Protein:
  • Superman Repression Domain X (SRDX) (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAGTTGGGTGATGAAGATGGTGT

Resource Information

• Supplemental Documents:
  • pKI_ΩBBMVP16&SRDXWUSm1.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry