pCherry-mNrp1
(Plasmid #21934)

• Depositing Lab: Guido Serini
• Publication: Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Backbone

• Vector backbone: pAcGFP1-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: neuropilin 1
• Alt name: Nrp; NP-1; Npn1; NPN-1; C530029I03; Nrp1
• Species: M. musculus (mouse)
• Insert Size (bp): 2769
• GenBank ID: NM_008737
• Entrez Gene: Nrp1 (a.k.a. C530029I03, NP-1, NPN-1, Npn1, Nrp)
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Cherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nrp1 cDNA was a gift from Dr. Andreas Püschel, Institut für Allgemeine Zoologie und Genetik Westfälische Wilhelms-Universität Münster
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AcGFP was substituted with Cherry from Roger Tsien's laboratory cloned between AgeI and NotI