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Addgene

pAL119-TK
(Plasmid #21911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAL119
  • Backbone size w/o insert (bp) 7337
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HSV Type 1 thymidine kinase
  • Alt name
    HSV1-TK
  • Alt name
    TK
  • Species
    Herpes Simplex Type 1
  • Insert Size (bp)
    1142
  • GenBank ID
    FJ655139

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Our diagnostic digest reveals a possible insertion within the backbone of this plasmid. It is unknown whether this discrepancy affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL119-TK was a gift from Maria Castro (Addgene plasmid # 21911 ; http://n2t.net/addgene:21911 ; RRID:Addgene_21911)

  • For your References section:

    Chronic brain inflammation and persistent herpes simplex virus 1 thymidine kinase expression in survivors of syngeneic glioma treated by adenovirus-mediated gene therapy: implications for clinical trials. Dewey RA, Morrissey G, Cowsill CM, Stone D, Bolognani F, Dodd NJ, Southgate TD, Klatzmann D, Lassmann H, Castro MG, Loewenstein PR. Nat Med. 1999 Nov . 5(11):1256-63. 10.1038/15207 PubMed 10545991
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