mCHOP10(dLZ).pCDNA1
(Plasmid #21900)

• Depositing Lab: David Ron
• Publication: Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10.1101/gad.6.3.439.

Backbone

• Vector backbone: pcDNA1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B/P3
• Growth instructions: needs P3-containing host strain such as DH10B/P3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHOP10
• Species: M. musculus (mouse)
• Mutation: The coding region of the murine CHOP-10 cDNA was interrupted at the unique Nhe1 site, making delta leucine zipper
• Entrez Gene: Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133.

Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.

Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.