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Addgene

boP58-pBabe.puro(notag)
(Plasmid #21885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    STBL2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P58
  • Alt name
    DNAJC3
  • Insert Size (bp)
    1652
  • Entrez Gene
    DNAJC3
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results show there is no HA tag in this plasmid.

The start codon of the gag gene has been mutated so that it does not get expressed. Its presence, however,increases the level of retroviral production.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    boP58-pBabe.puro(notag) was a gift from David Ron (Addgene plasmid # 21885 ; http://n2t.net/addgene:21885 ; RRID:Addgene_21885)

  • For your References section:

    Cotranslocational degradation protects the stressed endoplasmic reticulum from protein overload. Oyadomari S, Yun C, Fisher EA, Kreglinger N, Kreibich G, Oyadomari M, Harding HP, Goodman AG, Harant H, Garrison JL, Taunton J, Katze MG, Ron D. Cell. 2006 Aug 25. 126(4):727-39. 10.1016/j.cell.2006.06.051 PubMed 16923392
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