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pBEVY-pT231 scFv-GFP
(Plasmid #218827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCT
  • Backbone manufacturer
    Eric Boder
  • Backbone size w/o insert (bp) 6220
  • Total vector size (bp) 6958
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pT231 tau scFv
  • Alt name
    phospho-tau pT231 scFv
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    738
  • Promoter GAL1-10

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pT231 tau antibody gene was cloned by Heather Shih and colleagues reported in DOI 10.1074/jbc.M112.415935

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEVY-pT231 scFv-GFP was a gift from Yongku Cho (Addgene plasmid # 218827 ; http://n2t.net/addgene:218827 ; RRID:Addgene_218827)

  • For your References section:

    Yeast biopanning against site-specific phosphorylations in tau. Arbaciauskaite M, Pirhanov A, Ammermann E, Lei Y, Cho YK. Protein Eng Des Sel. 2023 Jan 21;36:gzad005. doi: 10.1093/protein/gzad005. 10.1093/protein/gzad005 PubMed 37294629
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