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pSSRB070_pAC8-hsCRBN
(Plasmid #218789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC8
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein cereblon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1458
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • Flag-TEV-Spy (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSSRB070_pAC8-hsCRBN was a gift from Eric Fischer (Addgene plasmid # 218789 ; http://n2t.net/addgene:218789 ; RRID:Addgene_218789)

  • For your References section:

    Continuous evolution of compact protein degradation tags regulated by selective molecular glues. Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, Deb A, Choudhary A, Fischer ES, Liu DR. Science. 2024 Mar 15;383(6688):eadk4422. doi: 10.1126/science.adk4422. Epub 2024 Mar 15. 10.1126/science.adk4422 PubMed 38484051
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