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Depositing Lab
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Publication
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 21874 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)KJ1C
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Growth instructionsWe recommend that it be streaked on an LB plate containing 12.5 µg/ml tetracycline. (Note that tetracycline stock can be made up at 12.5 mg/ml in 80% ethanol and stored at -20° C.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKJ1C bacterial Strain
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
F- ∆hisB463 ∆(gpt-proAB-arg-lac)XIII zaj::Tn10
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Strain KJ1C was a gift from Keith Joung (Addgene plasmid # 21874) -
For your References section:
Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nat Protoc. 2009. 4(10):1471-501. 10.1038/nprot.2009.98 PubMed 19798082