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peGFP C2-IST1
(Plasmid #218582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C2
  • Total vector size (bp) 5826
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IST1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1101
  • Entrez Gene
    IST1 (a.k.a. CHMP8, OLC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer peGFP-for
  • 3′ sequencing primer peGFP-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pinki Gahlot, Bojana Kravic, Giulia Rota, Johannes van den Boom, Sophie Levantovsky, Nina Schulze, Elena Maspero, Simona Polo, Christian Behrends, Hemmo Meyer,
Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH,
Molecular Cell,
2024

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peGFP C2-IST1 was a gift from Hemmo Meyer (Addgene plasmid # 218582 ; http://n2t.net/addgene:218582 ; RRID:Addgene_218582)

  • For your References section:

    Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH. Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H. Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029. 10.1016/j.molcel.2024.02.029 PubMed 38503285
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