pcDNA-Dest53-SHP-1 S591A
(Plasmid
#21802)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA-Dest53
- Backbone size w/o insert (bp) 7767
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHP-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1785
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Mutationchanged Serine 591 to Alanine
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Entrez GenePTPN6 (a.k.a. HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site gataway cloning (not destroyed)
- 3′ cloning site gataway cloning (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlasmids are derived from a construct originally from Dr. Taolin Yi
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Dest53-SHP-1 S591A was a gift from Stephen Shaw (Addgene plasmid # 21802 ; http://n2t.net/addgene:21802 ; RRID:Addgene_21802) -
For your References section:
Rapid T cell receptor-mediated SHP-1 S591 phosphorylation regulates SHP-1 cellular localization and phosphatase activity. Liu Y, Kruhlak MJ, Hao JJ, Shaw S. J Leukoc Biol. 2007 Sep . 82(3):742-51. 10.1189/jlb.1206736 PubMed 17575265
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/14/25/eee304d0-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)