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Addgene

Human FLAG-UAF1 S170Y
(Plasmid #217619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UAF1
  • Species
    H. sapiens (human)
  • Mutation
    S170Y
  • Entrez Gene
    WDR48 (a.k.a. Bun62, P80, SPG60, UAF1)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human FLAG-UAF1 S170Y was a gift from Ethan Lee (Addgene plasmid # 217619 ; http://n2t.net/addgene:217619 ; RRID:Addgene_217619)

  • For your References section:

    The USP46 complex deubiquitylates LRP6 to promote Wnt/beta-catenin signaling. Ng VH, Spencer Z, Neitzel LR, Nayak A, Loberg MA, Shen C, Kassel SN, Kroh HK, An Z, Anthony CC, Bryant JM, Lawson A, Goldsmith L, Benchabane H, Hansen AG, Li J, D'Souza S, Lebensohn AM, Rohatgi R, Weiss WA, Weiss VL, Williams C, Hong CC, Robbins DJ, Ahmed Y, Lee E. Nat Commun. 2023 Oct 5;14(1):6173. doi: 10.1038/s41467-023-41836-z. 10.1038/s41467-023-41836-z PubMed 37798301
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