pAAV-TetON-Bmpr1A-ca
(Plasmid #217536)

• Purpose: AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor
• Depositing Lab: Peter Scheiffele
• Publication: Okur et al bioRxiv 2023

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: constitutively active BMP receptor 1a
• Alt name: caBMPR1a
• Species: H. sapiens (human)
• Entrez Gene: BMPR1A (a.k.a. 10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5)
• Promoter: Tet operon (TetO)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: CACCACGCGAGGCGCGAGATA

Resource Information

• Supplemental Documents:
  • pAAV-TetON-Bmpr1A-ca.annotation.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry