Clathrin-LCa-ECFP
(Plasmid #21742)

• Depositing Lab: Xiaowei Zhuang
• Publication: Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):11790-5.

Backbone

• Vector backbone: pECFP-N1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Clathrin, light polypeptide (LCa)
• Alt name: Clta
• Species: M. musculus (mouse)
• Insert Size (bp): 657
• GenBank ID: NM_001080385
• Entrez Gene: Clta (a.k.a. Lca)
• Tag / Fusion Protein:
  • ECFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Age1 (not destroyed)
• 5′ sequencing primer: EGFP-N, Clonetech# 6479-1
• 3′ sequencing primer: EGFP-C, Clonetech# 6478-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gift of J.H. Keen, Nat. Cell Biol. 1,1,(1999)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry