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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePet21b
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepolyA polymerase
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Alt namePap1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1500
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Mutation30 amino acids off the c terminus
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Entrez GenePAP1 (a.k.a. YKR002W)
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Tag
/ Fusion Protein
- HIS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See attached purification scheme
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAP-delta10-cHIS was a gift from Andrew Bohm (Addgene plasmid # 21716 ; http://n2t.net/addgene:21716 ; RRID:Addgene_21716) -
For your References section:
Kinetic studies of yeast polyA polymerase indicate an induced fit mechanism for nucleotide specificity. Balbo PB, Meinke G, Bohm A. Biochemistry. 2005 May 31. 44(21):7777-86. 10.1021/bi050089r PubMed 15909992