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Addgene

T7-LacO-LgBiT
(Plasmid #216779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    ATUM
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LgBiT
  • Species
    Synthetic; O. gracilirostris
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-LacO-LgBiT was a gift from Alex Dunn (Addgene plasmid # 216779 ; http://n2t.net/addgene:216779 ; RRID:Addgene_216779)

  • For your References section:

    Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems. Zhong BL, Elliot JM, Wang P, Li H, Hall RN, Wang B, Prakash M, Dunn AR. ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7. 10.1021/acssensors.3c02664 PubMed 38973210
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