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pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
(Plasmid #216733)

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  • Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
  • Depositing Lab
    Vincent Dion
  • Publication
    Alvaro et al bioRxiv ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-D10A nickase and sgCTG
  • gRNA/shRNA sequence
    CTGCTGCTGCTGCTGCTGC
  • Species
    S pyogenes
  • Mutation
    D10A
  • Promoter U6 and CAG

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast was a gift from Vincent Dion (Addgene plasmid # 216733 ; http://n2t.net/addgene:216733 ; RRID:Addgene_216733)

  • For your References section:

    Cas9 nickase-mediated contraction of CAG/CTG repeats at multiple disease loci. Alvaro Murillo, Melanie Alpaugh, Meghan Larin, Emma L. Randall, Laura Heraty, Ruban Rex Durairaj, Alys N. Aston, Alysha S. Taylor, Alex Mas Monteys, Nina Stöberl, Aeverie E. R. Heuchan, Pascale Aeschlimann, Soumyasree Bhattacharyya, Nicholas D. Allen, Jack Puymirat, Beverly L. Davidson, Francesca Cicchetti, Mariah Lelos, Vincent Dion. bioRxiv 10.1101/2024.02.19.580669
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