pLV(gRNA)-CMV-eGFP-U6(sgCTG)
(Plasmid
#216732)
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PurposeContains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Backbone manufacturerVectorBuilder
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgCTG lentiviral guide RNA with eGFP tag
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SpeciesSynthetic
- Promoter CMV and U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV(gRNA)-CMV-eGFP-U6(sgCTG) was a gift from Vincent Dion (Addgene plasmid # 216732 ; http://n2t.net/addgene:216732 ; RRID:Addgene_216732) -
For your References section:
Cas9 nickase-mediated contraction of CAG/CTG repeats at multiple disease loci. Alvaro Murillo, Melanie Alpaugh, Meghan Larin, Emma L. Randall, Laura Heraty, Ruban Rex Durairaj, Alys N. Aston, Alysha S. Taylor, Alex Mas Monteys, Nina Stöberl, Aeverie E. R. Heuchan, Pascale Aeschlimann, Soumyasree Bhattacharyya, Nicholas D. Allen, Jack Puymirat, Beverly L. Davidson, Francesca Cicchetti, Mariah Lelos, Vincent Dion. bioRxiv 10.1101/2024.02.19.580669
