pET151-D-TOPO-PARP14-SΔK
(Plasmid #216658)

• Purpose: Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
• Depositing Lab: Herwig Schüler
• Publication: Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580. doi: 10.1016/j.pep.2024.106580.

Backbone

• Vector backbone: pET151-D-TOPO
• Backbone size w/o insert (bp): 5757
• Total vector size (bp): 8964
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence)
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3207
• Mutation: Deletion starting from Q1390 to D1448 and substitution with SUMO-TEV sequence
• GenBank ID: Q460N5 (UniProt)
• Entrez Gene: PARP14 (a.k.a. ARTD8, BAL2, PARP-14, pART8)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-V5-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET151-D-TOPO-PARP14-SΔK.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry