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pET151-D-TOPO-PARP14-SΔK
(Plasmid #216658)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151-D-TOPO
  • Backbone size w/o insert (bp) 5757
  • Total vector size (bp) 8964
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3207
  • Mutation
    Deletion starting from Q1390 to D1448 and substitution with SUMO-TEV sequence
  • GenBank ID
    Q460N5 (UniProt)
  • Entrez Gene
    PARP14 (a.k.a. ARTD8, BAL2, PARP-14, pART8)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-V5-TEV (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-D-TOPO-PARP14-SΔK was a gift from Herwig Schüler (Addgene plasmid # 216658 ; http://n2t.net/addgene:216658 ; RRID:Addgene_216658)

  • For your References section:

    A multidomain PARP14 construct suitable for bacterial expression. Chatzicharalampous C, Schuler H. Protein Expr Purif. 2024 Aug 16:106580. doi: 10.1016/j.pep.2024.106580. 10.1016/j.pep.2024.106580 PubMed 39154924
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