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Addgene

pEGFP-Rubicon
(Plasmid #21636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C2 (Clontech)
  • Backbone size w/o insert (bp) 4727
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rubicon
  • Alt name
    KIAA0226
  • Alt name
    Riken
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2826
  • Mutation
    *(see below)
  • GenBank ID
    AK030368
  • Entrez Gene
    1700021K19Rik (a.k.a. Rubicon, Kiaa0226, mKIAA0226, 5330403K09)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note: Compared to the Entrez Genbank reference sequence, Addgene's quality control sequencing finds an L617P amino acid residue substitution. The affect of this residue change is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-Rubicon was a gift from Tamotsu Yoshimori (Addgene plasmid # 21636 ; http://n2t.net/addgene:21636 ; RRID:Addgene_21636)

  • For your References section:

    Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Matsunaga K, Saitoh T, Tabata K, Omori H, Satoh T, Kurotori N, Maejima I, Shirahama-Noda K, Ichimura T, Isobe T, Akira S, Noda T, Yoshimori T. Nat Cell Biol. 2009 Apr . 11(4):385-96. 10.1038/ncb1846 PubMed 19270696
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