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Addgene

pTOPO-MCL1S159A
(Plasmid #21606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 /V5-His-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCL-1 S159A
  • Alt name
    BCL2L3
  • Alt name
    NM_021960
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1052
  • Mutation
    Serine 159 mutated to Alanine, abolishing phosphorylation by GSK-3. Insert ends with a STOP codon, no V5-His-tag.
  • GenBank ID
    NM_021960
  • Entrez Gene
    MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site T/A (destroyed during cloning)
  • 3′ cloning site T/A (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTOPO-MCL1S159A was a gift from Ulrich Maurer (Addgene plasmid # 21606 ; http://n2t.net/addgene:21606 ; RRID:Addgene_21606)

  • For your References section:

    Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1. Maurer U, Charvet C, Wagman AS, Dejardin E, Green DR. Mol Cell. 2006 Mar 17. 21(6):749-60. 10.1016/j.molcel.2006.02.009 PubMed 16543145
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