pCMV5-FLAG-MKK6-K82M
(Plasmid
#21583)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCMV5
-
Backbone manufacturerDr. David Russell, UTSW
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMKK6
-
Alt nameMEK6
-
SpeciesH. sapiens (human)
-
Mutationlysine 82 to methionine
-
Entrez GeneMAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
-
Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
M2-FLAG,
Lysine mutant, inactive
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV5-FLAG-MKK6-K82M was a gift from John Kyriakis (Addgene plasmid # 21583 ; http://n2t.net/addgene:21583 ; RRID:Addgene_21583) -
For your References section:
Tumor necrosis factor signaling to stress-activated protein kinase (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 1 and SAPK while receptor interacting protein associates with a mitogen-activated protein kinase kinase kinase upstream of MKK6 and p38. Yuasa T, Ohno S, Kehrl JH, Kyriakis JM. J Biol Chem. 1998 Aug 28. 273(35):22681-92. 10.1074/jbc.273.35.22681 PubMed 9712898
Map uploaded by the depositor.
